ssh

NOS bro!

If I was doing a big enduro trip I wouldn't trust them, probably go with a KTM.

Really? A few of my buddies who ride cross country love them. They ride enduro style bikes though... Yeah my father rides with a group that primarily ride BMW and they're always having shit break on their bikes, doesn't matter if it's a few years old or brand new. They all seem pretty content with them despite the fact.

I don't know about the cars, but BMW motorcycles are the biggest pieces of shit.

Headaches suck! As a kid I would get them so bad I would throw up.. Same here, mine were induced by allergies. I was allergic to everything under the sun.

DO YOU HAVE A PET LION?

Ever heard of context? Fuck.

Gonna burn the fuck outta some steaks? I kid. Any plans? Or a lazy Sunday?

No shit.Did I tell you the argument I got into with roommate Sean over eggs and the fact that whites don't even need to solidify to kill salmonella? A specialist at the federal government told him in a class that runny yolks could have serious health concerns. My head exploded. Bacteria need biotin. Egg whites have an enzyme called Avidin that has an incredibly high binding affinity for biotin, so the bacteria can't use it. Good stuff! Always enjoy your technical awnsers Stephan. What's even more interesting is how we exploit it today for protein purification. Say you want to study a specific enzyme, so you insert the genes for the enzyme in E.coli, grow the cells so it makes the enzyme then lyse them (tear open cell walls exposing all enzymes, DNA, RNA, etc), but now the solution has a bunch of different shit in it, not just the enzyme you want to study. You can do a bunch of purification steps by exploiting the differences in size, shape, charge, and solubility of the enzymes (as well as temperature and pH), but this takes a lot of time and has a lower yield. Another option is the portion of Avidin that binds to biotin has been identified (called strep-tag now), and that binding portion can be added to the enzyme you want to study, so now you have a gene for this modified enzyme that binds to biotin. Insert that gene in E.coli, grow the cells, lyse them, now you have your modified enzyme as well as everything else in solution. Run the solution through a column that has resin with biotin, everything will pass through the column except for the modified enzyme as it'll bind to the biotin. Then run an elution solution through the column to bring the modified enzyme off and use another enzyme to cleave the strep-tag off if needed. The interaction between Avidin and biotin is too strong in fact to separate the two once they bind (without destroying the enzyme in the process), so they actually use analogs of biotin with a lower binding affinity, but now you can separate a single type of enzyme from hundreds of other enzymes in one single step with high yield and little time. There's a bunch of other ways, but it's pretty cool what we discover in nature and exploit for other uses.

Surprisingly no. Morels are the big one to hunt around here. Local restaurants will buy them up in a heartbeat. Feel like a rude prick for no asking in return. Any shroom hunting for you? Nope lol. I read an article a while back about the cost of truffles and people going out with their dogs to find them. No mushroom hunting either, that seems too easy to pick the wrong ones, and I don't want to pay that price.

No shit. Did I tell you the argument I got into with roommate Sean over eggs and the fact that whites don't even need to solidify to kill salmonella? A specialist at the federal government told him in a class that runny yolks could have serious health concerns. My head exploded. Bacteria need biotin. Egg whites have an enzyme called Avidin that has an incredibly high binding affinity for biotin, so the bacteria can't use it.

I like the 4 ingredient rule. :-) I'll try it. Not sure if I'm willing to give up carbonated stimulants or protein powders as a supplement.... How do you carbonate amphetamines?

Ever done any truffle hunting?

Fuck yeah! Ironically I haven't eaten much butter in a while.

Eating burnt shit isn't healthy either.

I kinda forgot steak sauce was even a thing.

That is fucking spooky, not that I clicked on the link but I've done enough consulting at Medtronic and Guidant CPI to know exactly what could happen... It's worse too, after they connected to one within range (50ft ot 50m I don't remember) they were able to figure out how to connect back to the manufacturer's server and from there connect to other pacemakers (be it across the world).

Not car related, but just as interesting, there was a group that remotely took control over pacemakers. http://www.computerworld.com/article/2492453/malware-vulnerabilities/pacemaker-hack-can-deliver-deadly-830-volt-jolt.html

That makes me wonder who got hard when I shared my meat pics? Just for you buddy: http://www.reddit.com/r/shittyfoodporn

ROFL. Bunch of funny videos, but that one really had me giggling. At first I thought he was serious and I was thinking "these fucking people.."

ROFL.

They both taste like shit from teh store. Oh, difference.. meh, I don't eat PB.I cant peanut butter my dick in your ass I thought you were going to say you couldn't put one on your dick for your dog to lick off.

That's crazy, you lifting in the artic?

Biggest problem has been my lack of appetite and getting enough food in, but I'm starting to overcome that.